Systems Biology Imaging
Andrew Woehler
Research & Events
In addition to supporting the work of other researchers within the BIMSB and MDC, we also conduct research investigating the structure, function, and modulation of the neuronal synapse.
Research Interests
Total internal reflection fluorescence microscopy reveals the activity of primary hippocampal neurons. Data was acquired by Dr. Zohreh Farsi.
We are working to adapt existing or developing novel technology including:
- Small molecule and genetically encoded biosensors
- Experimental multiplexing techniques
- Advanced image analysis tools
to quantitatively study intracellular signal transduction and synaptic transmission. We aim at contributing to a better understand how information is processed at subcellular, cellular, and networks scales and how this processing is altered in neurological disorders.
Events
Upcoming Events
Microscopy Club | monthly
Previous Events
11th Berlin Summer Meeting and BIMSB Opening Symposium | 25-27 October 2018
Leica workshop on Multiphoton Confocal and Advanced Live Cell Imaging | 19-22 September 2017
MDC Technology Training in Light Microscopy / Zeiss workshop | 11-13 July 2017
10th Berlin Summer Meeting "Imaging Gene Regulation from DNA to RNA to protein” | 8-10 June 2017
EU-Life Course Biological Image Reconstruction and Analysis | 14-18 November 2016
Team
Equipment
Leica SP8 SMD confocal
microscope equipped with 4 hybrid detectors, 1 PMT, 405 nm near-UV diode laser, 440 nm pulsed laser, white light laser, and environmental control chamber.
TIRF / Wide-field microscope
based on a Nikon TiE with an iLas 2 TIRF
360 degree spinning uniform field TIRF (with simultaneous FRAP, FLIP, photoactivation), and a Prime-95B back-illuminated sCMOS
STORM setup
built on a Zeiss Observer Z.1
equipped with custom flat-field illumination and 3D multicolor spectral emission optics, and an sCMOS camera
Wide-field FRET microscope
built on a Nikon TE2000
equipped with an OptoSplit III and an EMCCD
Manufacturing Lab
- Ultimaker2 Extended 3D printer
- Form2 3D printer
- Various tools
Publications
News
Training & Resources
Training
Theoretical and hands on training required for all systems (est. 2 hrs).
Booking
Go to https://mdc.science-it.ch/ to register for our booking system. After registering, go to the browse tab, then resources, search for the instrument you are interested in using (i.e. SP8 SMD), click on the blue arrow under the device picture to request access. The platform will get an email and contact you to schedule a training session. Afterwards which we will grant you access and you can book the system anytime you like.
Resources
Refractive Index Matching
It is important to choose your imaging objective such that its refractive index matches as closely as possible that of your imaging mounting media
| Water | 1.33 |
|---|---|
| Silicone | 1.406 |
| Glycerol | 1.47 |
| Oil | 1.518 |
| 75% Glycerol | 1.44 | |
|---|---|---|
| Vectashield | 1.457 | |
| Mowiol | 1.49 | |
| ProLong Gold |
Fresh mounting Cured for 1 day Cured for 4 days |
1.39 1.40 1.44 |
Useful Links
