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The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites

Authors

  • M. Grzybek
  • A. Golonko
  • A. Górska
  • K. Szczepaniak
  • A. Strachecka
  • A. Lass
  • P. Lisowski

Journal

  • Applied Microbiology and Biotechnology

Citation

  • Appl Microbiol Biotechnol 102 (11): 4629-4640

Abstract

  • The CRISPR/Cas9 system, a natural defence system of bacterial organisms, has recently been used to modify genomes of the most important protozoa parasites. Successful genome manipulations with the CRISPR/Cas9 system are changing the present view of genetics in parasitology. The application of this system offers a major chance to overcome the current restriction in culturing, maintaining and analysing protozoan parasites, and allows dynamic analysis of parasite genes functions, leading to a better understanding of pathogenesis. CRISPR/Cas9 system will have a significant influence on the process of developing novel drugs and treatment strategies against protozoa parasites.


DOI

doi:10.1007/s00253-018-8927-3