Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
Authors
- Z. Izsvak
- J. Froehlich
- I. Grabundzija
- J.R. Shirley
- H.M. Powell
- K.M. Chapman
- Z. Ivics
- F.K. Hamra
Journal
- Nature Methods
Citation
- Nat Methods 7 (6): 443-445
Abstract
Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.