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Advanced Light Microscopy

Profil

Aim of the Advanced Light Microscopy (ALM) technology platform is to give researchers from the MDC, the BIMSB and the ECRC on the campus Berlin-Buch access to a broad range of high-end, state-of-the-art imaging techniques and to provide scientific and methodological support to enable them to perform advanced imaging experiments and to acquire high quality microscopic images from different specimens ranging from fixed cells and tissue sections to live cells, tissues and modell organisms.

Available microscopy techniques include

  • confocal laser scanning microscopy
  • two-photon microscopy
  • light-sheet microscopy
  • wide-field fluorescence microscopy
  • TIRF microscopy
  • stimulated emission depletion (STED) superresolution microscopy
  • fluorescence lifetime microscopy (FLIM)
  • laser-assisted microdissection and catapulting

As part of collaborative projects ALM offers expertise to find optimal image acquisition and data analysis tools for advanced imaging experiments, i.e. by consultation about experimental design and specimen preparation, the optimisation of image acquisition conditions and customised image processing and analysis workflows.

We also contribute to the establishment of new imaging methods, i.e., light-sheet microscopy, functional imaging (FLIM-FRET), live/intravital microscopy or image analysis.

Membership and funding

ALM of the MDC is member of the steering committee and actively contributes to the GermanBioImaging, a network of german microscopists and imaging specialists. http://www.germanbioimaging.org/wiki/index.php/Main_Page

ALM is co-funded by DFG, BIH and DZHK.

Deutsche ForschungsgemeinschaftBerlin Institute of HealthDZHK

Publications

Image Analysis & Data Processing

Advanced Light Microscopy Platform also provides access to several high-end computer workstations with different image processing and analysis software packages and support with image analysis and data processing for, i.e.:

  • Stitching
  • Deconvolution
  • 3D Reconstructions
  • Filament tracing
  • Quantitative colocalisation
  • Particle tracking

For advanced level data analysis tasks, including custom-designed software and macro-programming for users, please contact us.

Available Software packages

 

  • Zen software (for analysis of Zeiss data files )

  • LASAF software (for analysis of Leica data files) 

  • Imaris (Bitplane) for 3D reconstructions and analysis

  • Arivis for analysis and stitching of large image files

  • Huygens (SVI) for deconvolution

  • ImageJ/Fiji