Autoren der Studie

A close-up look at mutated DNA in cancer cells

PCAWG, the largest cancer research consortium in the world, has set itself the task of improving our understanding of genetic mutations in tumors. A new study by the international research group, to which the MDC substantially contributed, is now being published in the journal Nature.

No two tumors are alike. That’s why two people with the same kind of cancer can react very differently to the same medicine. In one the tumor gets smaller, in another the degenerated tissue remains unaffected. Usually this is due to genetic variations in the individual cancer cells.

In order to understand these variations in detail and better adapt therapies to the individual needs of patients in the future, an international team of more than 1,500 scientists – the Pan-Cancer Analysis of Whole Genomes (PCAWG) consortium – decided several years ago to trace the common mutation patterns in the genomes of about 3,000 cancer patients. The donated tissue samples included tumors of the liver, pancreas, brain, and 17 other organs.

Roland Schwarz (left), Julia Markowski (center) and Matthew Robert Huska (right) from the MDC worked on the paper.

PCAWG researchers used data from the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA) for their project. Participants in those groups had already completely sequenced the genomes and transcriptomes, that is the entire set of RNA molecules, of 1,188 degenerated tissue samples. 

Regulatory sequences of DNA were also decoded 

“Thanks to PCAWG, not only have the parts of genomes containing the blueprints for proteins from such a large number of patient samples been studied for the first time, but so have the regulatory sequences of DNA that control the expression of genes,” says Dr. Roland Schwarz, head of the Evolutionary and Cancer Genomics Group at the Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association. Schwarz is one of seven senior authors of the study in the current issue of Nature. This publication is the main paper of PCAWG Working Group 3 “Integration of Transcriptome and Genome.” Two scientists from Schwarz’s own research group at the Berlin Institute for Medical Systems Biology (BIMSB) at the MDC – Dr. Matthew Robert Huska and Julia Markowski – also contributed to the paper.

“In our work, we and our colleagues systematically studied a variety of RNA alterations and identified mutations on the DNA level that cause these RNA alterations,” Schwarz explains. Cancer-specific alterations on the RNA level have long been known. “Genes often get overexpressed in tumors, causing the formation of more RNA,” says Schwarz. “Or there are gene fusions or modifications in splicing, an important step in the formation of RNA.” In total, the PCAWG publishes more than 20 studies simultaneously in Nature, Nature Genetics, Nature Communications, Nature Biotech and Communications Biology.

Structural alterations in the genome play a decisive role

Until now, however, it has been rather unclear which DNA mutations are caused by the known RNA alterations. The work done by Schwarz and 49 other researchers has filled a significant gap in geneticists’ understanding of carcinogenesis.

An especially important focus of our work at the MDC has been on the interplay between selective DNA mutations and structural alterations, such as gene copy number variants
Roland Schwarz Senior Author of the paper

“An especially important focus of our work at the MDC has been on the interplay between selective DNA mutations and structural alterations, such as gene copy number variants,” Schwarz reports. “Our cells normally contain two inherited copies of each and every chromosome, a maternal and a paternal allele.” In cancer cells, the number of copies is often much higher, or entire segments of the genome have gone missing.”

Discussing different ways of testing somatic and germline single nucleotide variants for their effect on gene expression and regulation.

Using high-performance computers and cloud computing, Schwarz and his team have found out that such alterations in copy number play a decisive role in the altered gene expression of cancer cells. “By analyzing the DNA sequences of the entire genome, we have been able to accurately determine how copy number variants work together with point mutations – and what influence this in turn has on the expression of a specific parental allele,” Schwarz explains. 

Every one of a cell’s three billion base pairs can mutate

Schwarz hopes that the work he and his colleagues are doing will significantly improve our understanding of gene regulation in tumors. “Our study will certainly lay the foundation for further research that will help us to become more familiar with the genetic heterogeneity of tumors,” he says. 

For the fact is that every single one of the approximately three billion base pairs contained in a human genome could theoretically mutate. “To find out how cells actually degenerate, we have to distinguish between significant and insignificant mutations,” Schwarz says. And that’s only possible with a large number of tumor samples. And with a large number of dedicated researchers, such as we see united in the PCAWG consortium. The main paper the group plans to publish will certainly be of major interest.

Text: Anke Brodmerkel

Weiterführende Informationen 

Anmerkung für Journalisten: Bitte beachten Sie auch die Pressemitteilung der PCAWG, die ebenfalls unter Embargo verbreitet wird.

Literatur 

Claudia Calabrese et al. (2020): “Genomic basis for RNA alterations in cancer”, Nature, DOI: 10.1038/s41586-020-1970-0. 

 

Contacts

Dr. Roland Schwarz

Head of the lab “Evolutionary and Cancer Genomics“

Berlin Institute for Medical Systems Biology (BIMSB) of Max Delbrück Centrum for Molecular Medicine (MDC) 

+49-30-9406 3200

roland.schwarz@mdc-berlin.de

 

Christina Anders

Editor, communications department 

Max Delbrück Center for Molecular Medicine (MDC) 

+49-30-9406 2118 

christina.anders@mdc-berlin.de  oder presse@mdc-berlin.de

 

The Max Delbrück Center for Molecular Medicine (MDC)

 

The Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC) was founded in Berlin in 1992. It is named for the German-American physicist Max Delbrück, who was awarded the 1969 Nobel Prize in Physiology and Medicine. The MDC's mission is to study molecular mechanisms in order to understand the origins of disease and thus be able to diagnose, prevent and fight it better and more effectively. In these efforts the MDC cooperates with the Charité - Universitätsmedizin Berlin and the Berlin Institute of Health (BIH ) as well as with national partners such as the German Center for Cardiovascular Research and numerous international research institutions. More than 1,600 staff and guests from nearly 60 countries work at the MDC, just under 1,300 of them in scientific research. The MDC is funded by the German Federal Ministry of Education and Research (90 percent) and the State of Berlin (10 percent), and is a member of the Helmholtz Association of German Research Centers. www.mdc-berlin.de