Poulet Lab

Poulet Lab

Neural Circuits and Behaviour

Profile

We study sensory processing and perception in vivo to understand healthy brain function. The mouse forepaw sensorimotor system serves us as a model to understand the cellular mechanisms underlying the integration of sensory and motor signals in the brain.

We address these questions by a combination of techniques including two-photon microscopy, optogenetics, electrophysiology, and behavioral training.

We focus on brain regions associated with sensing and movement of the mouse forepaw, with a special focus on neocortical circuits. To investigate the link between neural activity and sensory perception we record and manipulate genetically identified neurons in mice performing trained behavioural tasks with their forepaw.

Our lab is a member of the Max Delbrück Center and the NeuroCure Cluster of Excellence and located at the Neuroscience Research Center (NWFZ), Campus Charité Mitte, Charitéplatz 1, 10117 Berlin.

Team

Research

Mission

The goal of our lab is to understand principles by which neural circuits process sensory information and guide adaptive motor behaviour.

Approach

We study the mouse as it is a mammalian, genetic model system where neurons can be recorded and their activity manipulated during quantified behaviour.

Methods

We focus our efforts on cortical regions associated with tactile sensing and movement of the mouse forepaw. We combine behavioural training with in vivo electrophysiological recording techniques (especially whole-cell patch clamp), 2-photon microscopy, optical techniques for manipulating neural activity and anatomical reconstruction of stained neurons.

Experiments

Our experiments are carried out in a brain jungle called the neocortex. We hope to cut paths in this jungle by targeting our experiments to small cortical regions, identifiable from mouse to mouse, and then using electrophysiological, genetic and optical techniques to record and manipulate neuronal activity at the synaptic, cellular and circuit level.  We often make whole-cell patch clamp recordings to be able to measure both the synaptic input and the spiking output of a cortical neuron to try to understand what sort of computations they perform.

We think it is important to link the anatomy of neurons to their function. During our experiments we therefore stain the neurons we have recorded to identify both their anatomical location in the brain and the cell type.  

Animals process sensory information when they are awake and behaving. We are therefore particularly interested in studying sensory perception during behaviour and the link between sensory and motor circuits.

In vivo, cortical neurons are always active. We would like to know how this activity is regulated, what it´s impact is on neural processing and how it ultimately leads to behaviour.

Publications

Dr. James Poulet
Dr. James Poulet
Group Leader
Contact
Phone: (030) 450 639 795
 
Secretariat
Janin Bergmann
Phone: (030) 450 539 818
 
Max-Delbrück-Centrum für Molekulare Medizin
Robert-Rössle-Str. 10
13125 Berlin

NWFZ - Charité - Campus Mitte
Charitéplatz 1
(Hufelandweg 14)
10117 Berlin

Research Topics