- A. Marg
- H. Haase
- T. Neumann
- M. Kouno
- I. Morano
- Biochemical and Biophysical Research Communications
- Biochem Biophys Res Commun 401 (1): 143-148
The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using Atomic Force Microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1-/- fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle.