Dr. Oliver Daumke

Max-Delbrück-Center for Molecular Medicine (MDC)

Max Delbrück House (Flachbau, 31.2), Room: 0225

Robert-Rössle-Str. 10

13125 Berlin, Germany

Phone: +49 30 9406 3425

oliver.daumke [at] mdc-berlin.de

www.mdc-berlin.de/daumke

 

G-proteins in membrane remodelling

Guanine nucleotide binding proteins (G-proteins) are involved in a diverse range of cellular processes including protein synthesis, sensual perception, vesicular transport and signal transduction cascades. Whereas small G-proteins are molecular switches that cycle between an active GTP-bound form and an inactive GDP-bound form, large G-proteins of the dynamin superfamily are mechano-chemical enzymes that use the energy of GTP hydrolysis to actively remodel membranes. Members of both groups bind to membranes, and this interaction is crucial for their function. Our projects aim to elucidate the interaction and reciprocal modulation of membranes and G-proteins using structural, biochemical and cell-biological methods.

 

Professional experience

Since 2008 | Helmholtz University Junior Group Leader at the Max-Delbrück Centrum for Molecular Medicine, Berlin

2004 - 2007 | Postdoctoral Fellow at the Laboratory of Molecular Biology (Cambridge, UK) with HT McMahon

Currently, my group consists of 1 PostDoc, 5 PhD students and 2 technical assistants and I have no teaching obligations.

 

Education/ Training

2001-2004 | PhD Studies at the Max-Planck Institute in Dortmund with A. Wittinghofer: “Structural and functional analysis of Rap1GAP” (summa cum laude)

2000     Diplomarbeit at the University of Cologne (very good)

1995-2000 | Studies in Biology at the Universities of Freiburg, Sussex (UK) and Cologne

 

Publications

Selbach, M, Paul FE, Brandt S, Guye, P, Daumke, O, Backert, S, Dehio, C, Mann M. (2009) Host cell interactome of tyrosine-phosphorylated bacterial proteins. Cell Host Microbe, 5, 397-403

 

Daumke, O, Lundmark R, Vallis, Y, Martens, S, Butler, PJ, McMahon HM. (2007) Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling. Nature, 449, 923-927.

 

Henne, WM, Kent, HM, Ford, MG, Hegde, BG, Daumke, O, Butler, PJ, Mittal, R, Langen, R, Evans, PR, McMahon, HT. (2007) Structure and analysis of FCHo2 F-BAR domain: A dimerizing and membrane recruitment module that effects membrane curvature. Structure, 15, 839-852.

 

Kupzig, S, Deaconescu, D, Bouyoucef, D, Walker SA, Liu Q, Polte, CL, Daumke, O, Ishizaki, T, Lockyer, PJ, Wittinghofer, A, Cullen PJ. (2006) GAP1 family members constitute bifunctional RAS and RAP GTPase-activating proteins. J Biol Chem, 281, 9891-9900.

 

Daumke, O, Weyand , M, Chakrabarti PP, Vetter I, Wittinghofer A. (2004). The GTPase  activating protein Rap1GAP uses a catalytic asparagine. Nature, 429, 197-201