Daumke Lab

Daumke Lab

Structural Biology of Membrane-Associated Processes

GTPases of the dynamin and septin superfamilies are molecular machines that assemble at the surface of cellular membranes.

Some of these proteins act as dynamic scaffolds that remodel the underlying membrane in a GTPase-dependent fashion; others orchestrate the recruitment of interaction partner in a temporally and spatially defined manner.

We are interested to understand principles of assembly, function and regulation of these GTPases. To this end, structural studies are combined with biochemical and cell-based approaches.

Team

Group Leader

Prof. Dr. Oliver Daumke
Prof. Dr. Oliver Daumke
31.2: Max-Delbrück-Haus
Room: 0262
+49 30 9406-3263
31.2: Max-Delbrück-Haus
Room: 0225
+49 30 9406-3425

Scientist

Dr. Tobias Bock-Bierbaum
Dr. Tobias Bock-Bierbaum
31.2: Max-Delbrück-Haus
Room: 1216.5
+49 30 9406-3689
Dr. Katja Fälber
Dr. Katja Fälber
31.2: Max-Delbrück-Haus
Room: 0227
+49 30 9406-3830
Dr. Yvette Roske
Dr. Yvette Roske
31.2: Max-Delbrück-Haus
Room: 0223
+49 30 9406-2949
Dr. Julia Smirnova
Dr. Julia Smirnova
31.2: Max-Delbrück-Haus
Room: 0232
+49 30 94063426

Secretariat

Jeannine Engel
+49 30 9406-2337

Technical Assistants

Carola Bernert
Carola Bernert
31.2: Max-Delbrück-Haus
Room: 0269
Jeanette Schlegel
Jeanette Schlegel
31.2: Max-Delbrück-Haus
Room: 0265
+49 30 9406-3280

PhD student

Mohd Saif
Mohd Saif
31.2: Max-Delbrück-Haus
Room: 0226
+49 30 9406-3139
Elena Vazquez Sarandeses
Elena Vazquez Sarandeses
31.2: Max-Delbrück-Haus
Room: 0223
Marius Weismehl
Marius Weismehl
+49 30 9406-3275
31.2: Max-Delbrück-Haus
Room: 0228

Other

Publications

/ Nat Chem Biol

Development of selective inhibitors of phosphatidylinositol 3-kinase C2α

  • W.T. Lo
  • H. Belabed
  • M. Kücükdisli
  • J. Metag
  • Y. Roske
  • P. Prokofeva
  • Y. Ohashi
  • A. Horatscheck
  • D. Cirillo
  • M. Krauss
  • C. Schmied
  • M. Neuenschwander
  • J.P. von Kries
  • G. Médard
  • Be. Kuster
  • O. Perisic
  • R.L. Williams
  • O. Daumke
  • B. Payrastre
  • S. Severin
  • M. Nazaré
  • V. Haucke
/ Sci Immunol

A multimorphic mutation in IRF4 causes human autosomal dominant combined immunodeficiency

  • O. Fornes
  • A. Jia
  • H.S. Kuehn
  • Q. Min
  • U. Pannicke
  • N. Schleussner
  • R. Thouenon
  • Z. Yu
  • M. de Los Angeles Astbury
  • C.M. Biggs
  • M. Galicchio
  • J.A. Garcia-Campos
  • S. Gismondi
  • G. Gonzalez Villarreal
  • K.J. Hildebrand
  • M. Hönig
  • J. Hou
  • D. Moshous
  • S. Pittaluga
  • X. Qian
  • J. Rozmus
  • A.S. Schulz
  • A.T. Staines-Boone
  • B. Sun
  • J. Sun
  • S. Uwe
  • E. Venegas-Montoya
  • W. Wang
  • X. Wang
  • W. Ying
  • X. Zhai
  • Q. Zhou
  • A. Akalin
  • I. André
  • T.F.E. Barth
  • B. Baumann
  • A. Brüstle
  • G. Burgio
  • J.C. Bustamante
  • J.L. Casanova
  • M.G. Casarotto
  • M. Cavazzana
  • L. Chentout
  • I.A. Cockburn
  • M. Costanza
  • C. Cui
  • O. Daumke
  • K.L. Del Bel
  • H. Eibel
  • X. Feng
  • V. Franke
  • J.C.M. Gebhardt
  • A. Götz
  • S. Grunwald
  • B. Hoareau
  • T.R. Hughes
  • E.M. Jacobsen
  • M. Janz
  • A. Jolma
  • C. Lagresle-Peyrou
  • N. Lai
  • Y. Li
  • S. Lin
  • H.Y. Lu
  • S.O. Lugo-Reyes
  • X. Meng
  • P. Möller
  • N. Moreno-Corona
  • J.E. Niemela
  • G. Novakovsky
  • J.J. Perez-Caraballo
  • C. Picard
  • L. Poggi
  • M.E. Puig-Lombardi
  • K.L. Randall
  • A. Reisser
  • Y. Schmitt
  • S. Seneviratne
  • M. Sharma
  • J. Stoddard
  • S. Sundararaj
  • H. Sutton
  • L.Q. Tran
  • Y. Wang
  • W.W. Wasserman
  • Z. Wen
  • W. Winkler
  • E. Xiong
  • A.W.H. Yang
  • M. Yu
  • L. Zhang
  • H. Zhang
  • Q. Zhao
  • X. Zhen
  • A. Enders
  • S. Kracker
  • R. Martinez-Barricarte
  • S. Mathas
  • S.D. Rosenzweig
  • K. Schwarz
  • S.E. Turvey
  • J.Y. Wang
/ Nat Commun

Cryo-electron tomography reveals structural insights into the membrane remodeling mode of dynamin-like EHD filaments

  • A.A. Melo
  • T. Sprink
  • J.K. Noel
  • E. Vázquez-Sarandeses
  • C. van Hoorn
  • S. Mohd
  • J. Loerke
  • C.M.T. Spahn
  • O. Daumke
/ Sci Adv

Structural insights into crista junction formation by the Mic60-Mic19 complex

  • T. Bock-Bierbaum
  • K. Funck
  • F. Wollweber
  • E. Lisicki
  • K. von der Malsburg
  • A. von der Malsburg
  • J. Laborenz
  • J.K. Noel
  • M. Hessenberger
  • S. Jungbluth
  • C. Bernert
  • S. Kunz
  • D. Riedel
  • H. Lilie
  • S. Jakobs
  • M. van der Laan
  • O. Daumke
/ J Exp Med

GIMAP6 regulates autophagy, immune competence, and inflammation in mice and humans

  • Y. Yao
  • P. Du Jiang
  • B.N. Chao
  • D. Cagdas
  • S. Kubo
  • A. Balasubramaniyam
  • Y. Zhang
  • B. Shadur
  • A. NaserEddin
  • L.R. Folio
  • B. Schwarz
  • E. Bohrnsen
  • L. Zheng
  • M. Lynberg
  • S. Gottlieb
  • M.A. Leney-Greene
  • A.Y. Park
  • I. Tezcan
  • A. Akdogan
  • R. Gocmen
  • S. Onder
  • A. Rosenberg
  • E.J. Soilleux
  • E. Johnson
  • P.K. Jackson
  • J. Demeter
  • S.D. Chauvin
  • F. Paul
  • M. Selbach
  • H. Bulut
  • M.R. Clatworthy
  • Z.K. Tuong
  • H. Zhang
  • B.J. Stewart
  • C.M. Bosio
  • P. Stepensky
  • S. Clare
  • S. Ganesan
  • J.C. Pascall
  • O. Daumke
  • G.W. Butcher
  • A.J. McMichael
  • A.K. Simon
  • M.J. Lenardo
/ J Biol Chem

Competitively disrupting the neutrophil-specific receptor-autoantigen CD177:proteinase 3 membrane complex reduces anti-PR3 antibody-induced neutrophil activation

  • S.F. Marino
  • U. Jerke
  • S. Rolle
  • O. Daumke
  • R. Kettritz
/ Nat Struct Mol Biol

Structural basis of phosphatidylinositol 3-kinase C2α function

  • W.T. Lo
  • Y. Zhang
  • O. Vadas
  • Y. Roske
  • F. Gulluni
  • M.C. De Santis
  • A.V. Zagar
  • H. Stephanowitz
  • E. Hirsch
  • F. Liu
  • O. Daumke
  • M. Kudryashev
  • V. Haucke
/ Cell Res

SPFH protein cage - one ring to rule them all

  • O. Daumke
  • G.R. Lewin
/ Protein Sci

SMOG 2 and OpenSMOG: extending the limits of structure-based models

  • A.B. de Oliveira
  • V.G. Contessoto
  • A. Hassan
  • S. Byju
  • A. Wang
  • Y. Wang
  • E. Dodero-Rojas
  • U. Mohanty
  • J.K. Noel
  • J.N. Onuchic
  • P.C. Whitford
/ Nat Commun

Lysine acetylation regulates the interaction between proteins and membranes

  • A.K. Okada
  • K. Teranishi
  • M.R. Ambroso
  • J.M. Isas
  • E. Vazquez-Sarandeses
  • J.Y. Lee
  • A.A. Melo
  • P. Pandey
  • D. Merken
  • L. Berndt
  • M. Lammers
  • O. Daumke
  • K. Chang
  • I.S. Haworth
  • R. Langen

News

Scientific illustration
Science

Membrane couture made of elastic protein bands

For nearly two decades, Oliver Daumke has been working to solve the mystery of how a molecular machine remodels cellular membranes. His team has now found the answer with the help of cryo-electron tomography. They report their findings in the journal “Nature Communications”.
Mitochondrium
Science

How mitochondria stay in shape

Defective mitochondrial can lead to neurodegenerative and muscular diseases. But how does the “powerhouse” of the cell normally maintain its characteristic shape? In Science Advances, new molecular insights have now been provided by an MDC team led by Oliver Daumke.
Dynamin
Science

📺 A molecular motor for membrane constriction

The protein dynamin is a molecular machine that helps cells to take up nutrients and other substances. A team led by MDC researcher Oliver Daumke has now detailed how this minuscule motor works in the journal “PNAS”.
Das Team der Kryo-EM: Christoph Diebolder, Thiemo Sprink und Metaxia Stavroulaki
Institute & Campus

A small home for a big microscope

The new cryo-electron microscope on the Buch campus is four meters tall and captures images of tiny, nanometer-scale structures. The MDC has constructed a special building to house this feat of technology, which is now available to Berlin’s structural biology community. Dr. Christoph Diebolder of Charité heads the facility.
Kristall
Science

Getting to the heart of an enzyme

A team led by Oliver Daumke at the MDC has determined the three-dimensional structure of the NatC acetyltransferase in an article published in Nature Communications. This enzyme modifies cell proteins to enable them to perform their functions correctly. In cancer cells, however, NatC is often overactive.
header
Science By Christina Anders

Cells sound the alarm on chlamydia

A chlamydia infection triggers an inflammatory response at the cellular level. MDC researchers conducted experiments in cell cultures to examine the role that the enzyme GBP1 plays in this response. They have now published their findings in Cell Reports.
Lipidtropfen Fettzelle Adipocyte
Press Release No. 12 Berlin

Protein controls fat metabolism

A protein in the cell envelope influences the rate of fatty acid uptake in cells. This is reported by MDC researchers in the journal PNAS. This process also appears to be altered in overweight people.
Kryo Haus
Institute & Campus By Christina Anders

Buch campus to get ultralow temperature microscopes

By mid-2020 two new cryogenic electron microscopes (cryo-EM) will be available to scientists at the Buch campus. The building is almost finished, and the first crates of equipment were delivered in December. There’s still a lot to do, however, before everything is up and running.
PM Daumke Mitochondrien
Press Release No. 35 Berlin

Keeping a cell’s powerhouse in shape

A German-Swiss team around Professor Oliver Daumke from the MDC has investigated how a protein of the dynamin family deforms the inner mitochondrial membrane. The results, which also shed light on a hereditary disease of the optic nerve, have been published in Nature.
molecule model
Science

How cells devour themselves

Cells sometimes love themselves to pieces – and develop a big appetite for their own components. A group of researchers led by Professor Oliver Daumke of the MDC has now discovered how certain molecules trigger this self-cleaning process.
Prof. Dr. Oliver Daumke
Prof. Dr. Oliver Daumke
Contact
Phone: +49 30 9406-3425
Max-Delbrück-Centrum für Molekulare Medizin (MDC)
Robert-Rössle-Straße 10
13125 Berlin, Deutschland
Building 31.2, Room 0225

Research Topics