Generation of an induced pluripotent stem cell line from a Huntington's disease patient with a long HTT-PolyQ sequence


  • D.C. Miller
  • P. Lisowski
  • C. Genehr
  • E.E. Wanker
  • J. Priller
  • A. Prigione
  • S. Diecke


  • Stem Cell Research


  • Stem Cell Res 68: 103056


  • Huntington's disease (HD) is an inherited neurodegenerative disorder caused by an abnormal length of CAG repeats in the gene HTT, leading to an elongated poly-glutamine (poly-Q) sequence in huntingtin (HTT). We used non-integrative Sendai virus to reprogram fibroblasts from a patient with juvenile onset HD to induced pluripotent stem cells (iPSCs). Reprogrammed iPSCs expressed pluripotency-associated markers, exhibited a normal karyotype, and following directed differentiation generated cell types belonging to the three germ layers. PCR analysis and sequencing confirmed the HD patient-derived iPSC line had one normal HTT allele and one with elongated CAG repeats, equivalent to ≥180Q.