RC3H1 post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-κB pathway


  • Y. Murakawa
  • M. Hinz
  • J. Mothes
  • A. Schuetz
  • M. Uhl
  • E. Wyler
  • T. Yasuda
  • G. Mastrobuoni
  • C.C. Friedel
  • L. Dölken
  • S. Kempa
  • M. Schmidt-Supprian
  • N. Blüthgen
  • R. Backofen
  • U. Heinemann
  • J. Wolf
  • C. Scheidereit
  • M. Landthaler


  • Nature Communications


  • Nat Commun 6: 7367


  • The RNA-binding protein RC3H1 (also known as ROQUIN) promotes TNFα mRNA decay via a 3'UTR constitutive decay element (CDE). Here we applied PAR-CLIP to human RC3H1 to identify ~3,800 mRNA targets with >16,000 binding sites. A large number of sites are distinct from the consensus CDE and revealed a structure-sequence motif with U-rich sequences embedded in hairpins. RC3H1 binds preferentially short-lived and DNA damage-induced mRNAs, indicating a role of this RNA-binding protein in the post-transcriptional regulation of the DNA damage response. Intriguingly, RC3H1 affects expression of the NF-κB pathway regulators such as IκBα and A20. RC3H1 uses ROQ and Zn-finger domains to contact a binding site in the A20 3'UTR, demonstrating a not yet recognized mode of RC3H1 binding. Knockdown of RC3H1 resulted in increased A20 protein expression, thereby interfering with IκB kinase and NF-κB activities, demonstrating that RC3H1 can modulate the activity of the IKK/NF-κB pathway.