On the one hand, generation of complex spatial patterns proceeds with striking recision: the different tissues and organs are generally formed at the correct position, at the right time, and with a defined size. On the other hand, regulation should also not be too rigid, since embryos need to flexibly adjust to environmental perturbations and correct errors caused by noisy gene expression.
We study the interplay between variation and stability by using the zebrafish embryo as a model system. We aim to understand the stochastic mechanisms that underlie phenotypic diversity in isogenic populations, and to identify the error repair mechanisms that ensure robust pattern formation in the presence of perturbations. Systematic investigation of variability and robustness during vertebrate organ formation requires development of novel approaches for spatially-resolved gene expression profiling and linage analysis, ideally on the single cell level.
We have recently developed a method for spatially-resolved transcriptomics called 'tomo-seq', and we are currently working to expand this strategy to the level of lineage analysis. We use these approaches in combination with light microscopy and tools from zebrafish developmental biology. Our work combines biophysics, systems biology and developmental biology.