Protocol for efficient CRISPR/Cas9/AAV-mediated homologous recombination in mouse hematopoietic stem and progenitor cells

Autor/innen

  • N.T. Tran
  • J. Trombke
  • K. Rajewsky
  • V.T. Chu

Journal

  • STAR Protocols

Quellenangabe

  • STAR Protoc 1 (1): 100028

Zusammenfassung

  • Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse HSPCs. Here, we provide a step-by-step protocol to achieve high efficiency of gene knockin in mouse HSPCs, while maintaining engraftment capacity. This approach enables the functional study of hematopoietic disease mutations in vivo, without requiring germline mutagenesis.


DOI

doi:10.1016/j.xpro.2020.100028