BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples

Autor/innen

  • D. Hörl
  • F. Rojas Rusak
  • F. Preusser
  • P. Tillberg
  • N. Randel
  • R.K. Chhetri
  • A. Cardona
  • P.J. Keller
  • H. Harz
  • H. Leonhardt
  • M. Treier
  • S. Preibisch

Journal

  • Nature Methods

Quellenangabe

  • Nat Methods 16 (9): 870-874

Zusammenfassung

  • Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that consist of many unaligned three-dimensional image tiles, which must be reconstructed before analysis. We developed the BigStitcher software to address this challenge. BigStitcher enables interactive visualization, fast and precise alignment, spatially resolved quality estimation, real-time fusion and deconvolution of dual-illumination, multitile, multiview datasets. The software also compensates for optical effects, thereby improving accuracy and enabling subsequent biological analysis.


DOI

doi:10.1038/s41592-019-0501-0